Coding

Part:BBa_K3425043:Design

Designed by: Nuria Garriga Alonso   Group: iGEM20_UofUppsala   (2020-10-26)


B-F10: nanobody for HSP16.3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli.

This part is specifically designed for the agglutination construct of NANOFLEX with custom Type IIS fusion sites compatible with the iGEM Type IIS standard. Visit the Main page for more details.


Source

Sequence taken from Trilling, A. K., Ronde, H. de, Noteboom, L., Houwelingen, A. van, Roelse, M., Srivastava, S. K., Haasnoot, W., Jongsma, M. A., Kolk, A., Zuilhof, H., and Beekwilder, J. (2011) A Broad Set of Different Llama Antibodies Specific for a 16 kDa Heat Shock Protein of Mycobacterium tuberculosis. PLOS ONE. 6, e26754


References